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Položka č.:
104661774
Lab Buffer SYBR Safe DNA Stain
Položka č.:
104661774
CZK 2535
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SYBR Safe has been engineered to be less mutagenic than Ethidium Bromide, making it much safer to use, particularly in the classroom.
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What Stands Out
Safe Staining
SYBR Safe stain provides a safer alternative to ethidium bromide, minimizing health risks while still delivering high-quality visual results in DNA analysis.
High Sensitivity
Offers exceptional sensitivity to detect low quantities of DNA, ensuring precise results in experiments, which is crucial for accurate data in molecular biology.
Easy Handling
User-friendly formulation simplifies the staining process, enabling efficient use in labs, thereby enhancing workflow without compromising quality or safety.
Detaily produktu
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for those performing DNA gel electrophoresis, enhancing visualization without hazardous chemicals or UV exposure.
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Research Labs
Useful in academic and industrial research settings for sensitive detection of DNA in various applications.
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Educational Purposes
Great for educational institutions teaching molecular biology techniques with safer staining alternatives for students.
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High-Throughput Screening
Not suitable for situations requiring rapid and automated processing due to limited compatibility with robotic systems.
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In Vivo Applications
Not recommended for direct applications in living organisms as it has not been validated for in vivo use.
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Protein Staining
Ineffective for protein analysis, as this stain is specifically designed for nucleic acids rather than proteins.
POPIS PRODUKTU
Lab Buffer SYBR Safe DNA Stain
Máte nějaký dotaz? Napi
Otázky a odpovědi zákazníků
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otázka:
What is SYBR Safe DNA Stain used for?
Odpovědět: SYBR Safe DNA Stain is a sensitive fluorescent dye commonly used for visualizing DNA in agarose gels during gel electrophoresis. It is trusted for its high specificity and low background, allowing researchers to easily detect and analyze DNA fragments. The stain binds to DNA and fluoresces under UV or blue light, making it a vital tool in molecular biology and genetics laboratories. Depending on your application, such as checking DNA quality or quantifying PCR products, SYBR Safe provides reliable results for a wide range of experiments. -
otázka:
How does SYBR Safe DNA Stain compare to other DNA stains?
Odpovědět: Compared to traditional DNA stains like ethidium bromide, SYBR Safe DNA Stain offers a safer alternative, as it is less toxic and does not require harsh disposal protocols. Moreover, SYBR Safe has been shown to be more sensitive, allowing for the detection of lower concentrations of DNA. This means that whether you are working with plasmids, genomic DNA, or PCR products, SYBR Safe can deliver clearer, more reliable results with enhanced safety, making it an excellent choice for both experienced researchers and students. -
otázka:
Is SYBR Safe DNA Stain compatible with all types of gel electrophoresis?
Odpovědět: Yes, SYBR Safe DNA Stain is compatible with both agarose and polyacrylamide gel electrophoresis. This versatility makes it suitable for a variety of molecular biology applications, including RNA visualization. When preparing your gels, you can incorporate the stain directly into the gel matrix or stain the gel post-electrophoresis, depending on your experimental needs. Its compatibility ensures that you can use SYBR Safe across different types of experiments without compromising the quality of your results. -
otázka:
Can I use SYBR Safe DNA Stain for RNA visualization?
Odpovědět: SYBR Safe DNA Stain is primarily optimized for DNA visualization; however, it can also be used for RNA in certain applications. For best results, researchers typically use other stains specifically designed for RNA, but SYBR Safe can provide effective results when used in addressing RNA quality and integrity in electrophoresis. Be sure to verify your specific application requirements to ensure the best outcomes for your experiments involving RNA. -
otázka:
What safety precautions should I take when using SYBR Safe DNA Stain?
Odpovědět: While SYBR Safe DNA Stain is much safer than traditional DNA stains, it is still important to follow standard laboratory safety practices. Always wear gloves and safety goggles when handling the dye, and work in a well-ventilated area to minimize inhalation risk. Dispose of any contaminated materials according to local waste disposal regulations. These precautions will help maintain a safe working environment while maximizing the benefits SYBR Safe has to offer in your DNA analysis. -
otázka:
How do I properly store SYBR Safe DNA Stain?
Odpovědět: SYBR Safe DNA Stain should be stored at -20°C for long-term stability. Upon receipt, it is essential to aliquot the stain into smaller containers to prevent repeated freeze-thaw cycles that can compromise its effectiveness. Protect it from light during storage, and ensure that it is labeled clearly with the date of receipt to monitor its shelf life. Proper storage practices will help maintain the performance of the stain, ensuring high-quality results in your experiments. -
otázka:
Can I reuse gels stained with SYBR Safe DNA Stain?
Odpovědět: Generally, gels stained with SYBR Safe DNA Stain are not recommended for reuse due to potential contamination and diminished fluorescence. Each use can degrade the image quality and lead to inaccurate results. However, if you plan to take multiple images or analyze different DNA samples, consider running separate gel experiments to ensure the integrity of your results. This best practice ensures that each experiment yields reliable, reproducible data. -
otázka:
What is the recommended concentration of SYBR Safe DNA Stain for staining gels?
Odpovědět: For optimal results, SYBR Safe DNA Stain can be used at a final concentration of 0.5-2.0 µL per 100 mL of gel solution. Depending on your specific experimental goals, you might choose to adjust the concentration. For instance, if you need to visualize lower DNA concentrations or require enhanced sensitivity, you might opt for higher concentrations. It’s best to run a control for calibration to establish the ideal concentration for your specific assay. -
otázka:
Can I buy SYBR Safe DNA Stain in Czech Republic?
Odpovědět: Yes, you can buy SYBR Safe DNA Stain in Czech Republic from Ubuy. Ubuy offers a wide selection of laboratory supplies, including various brands and formats of SYBR Safe DNA Stain, ensuring that researchers will find what they need for their experiments. With reliable delivery options and favorable customer service, purchasing from Ubuy is a convenient choice for your laboratory needs.
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CZK 2535
Objednejte nyní a položka bude u vás okolo Monday, červenec 13
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Služby a výhody
- Fluoresces bright green under blue light.
- Specifically binds to the DNA double helix.
- Safer alternative to Ethidium Bromide.
- Designed for Biotechnology classrooms.
- 10,000 X Concentrate yields 750 ml.
- Volume of each use is just 80 µl.