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Položka č.:
114683255
10x Tris-HEPES-SDS Running Buffer 500 ML
Položka č.:
114683255
CZK 1550
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Objednejte nyní a položka bude u vás okolo Sobota, červenec 11
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Formulated for SDS-PAGE gel electrophoresis, ensuring precise and effective results.
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Detaily produktu
| Item Weight | 1.1 lbs (500 grams) |
POPIS PRODUKTU
10x Tris-HEPES-SDS Running Buffer 500 ML
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Otázky a odpovědi zákazníků
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otázka:
What is 10x Tris-HEPES-SDS Running Buffer used for?
Odpovědět: 10x Tris-HEPES-SDS Running Buffer is primarily used in electrophoretic methods such as SDS-PAGE for protein analysis. This buffer system provides the necessary ionic environment conducive to the separation of proteins based on their size. By maintaining a stable pH and providing a consistent charge, it allows scientists to run effective experiments, yielding high-resolution results. It’s particularly useful for assessing protein purity and molecular weight. -
otázka:
How do you dilute 10x Tris-HEPES-SDS Running Buffer for use?
Odpovědět: To prepare the working solution, dilute the 10x Tris-HEPES-SDS Running Buffer in deionized water at a 1:10 ratio, meaning one part buffer to nine parts water. This will yield a 1x working buffer, which is optimal for SDS-PAGE applications. Proper dilution is crucial as it ensures the buffer maintains its desired ionic strength and pH during electrophoresis, leading to accurate protein separation and analysis. -
otázka:
Can I use 10x Tris-HEPES-SDS Running Buffer for DNA gel electrophoresis?
Odpovědět: No, 10x Tris-HEPES-SDS Running Buffer is not suitable for DNA gel electrophoresis. This buffer is designed specifically for protein analysis, as SDS (Sodium Dodecyl Sulfate) denatures proteins by imparting a negative charge. For DNA electrophoresis, it is recommended to use buffers like TAE (Tris-Acetate-EDTA) or TBE (Tris-Boric Acid-EDTA) that provide the necessary conditions for nucleic acid separation without affecting the integrity of the DNA. -
otázka:
What types of experimental setups benefit from using 10x Tris-HEPES-SDS Running Buffer?
Odpovědět: Experimental setups that involve protein characterization, such as Western blotting and protein purification workflows, benefit significantly from using 10x Tris-HEPES-SDS Running Buffer. Its formulation ensures consistent results in protein separation, making it a preferred choice for studying protein interactions, purifying recombinant proteins, and validating protein expression levels in different samples. -
otázka:
Is 10x Tris-HEPES-SDS Running Buffer compatible with all types of proteins?
Odpovědět: While 10x Tris-HEPES-SDS Running Buffer is designed to work with a broad range of proteins, some specific proteins may be sensitive to denaturation caused by SDS. Methods like native PAGE, which do not require denaturing conditions, would not benefit from this buffer. It is important to consider the nature of the protein and the intended analysis to choose the appropriate buffer for optimal results. -
otázka:
How should 10x Tris-HEPES-SDS Running Buffer be stored?
Odpovědět: The buffer should be stored at room temperature in a tightly sealed container, away from direct sunlight. Avoiding contamination and ensuring a stable pH is crucial for maintaining the buffer’s effectiveness. Proper storage prolongs its shelf life, allowing researchers to achieve reliable results each time it is used in electrophoresis experiments. -
otázka:
What is the role of Tris in the 10x Tris-HEPES-SDS Running Buffer?
Odpovědět: Tris (tris(hydroxymethyl)aminomethane) acts as a buffering agent in 10x Tris-HEPES-SDS Running Buffer, helping to maintain a stable pH during electrophoresis. This stability is essential because variations in pH can affect protein charge and migration rates, leading to inconsistent results. By ensuring a constant pH, Tris facilitates accurate protein analysis in various experimental contexts. -
otázka:
Can 10x Tris-HEPES-SDS Running Buffer be reused?
Odpovědět: Generally, it is not advisable to reuse 10x Tris-HEPES-SDS Running Buffer after electrophoresis. After use, the buffer may contain proteins, SDS, and other contaminants that can compromise subsequent experiments. Fresh buffer preparation is recommended to maintain experiment integrity and reproducibility, especially in sensitive assays like Western blotting and mass spectrometry. -
otázka:
What precautions should be taken when using 10x Tris-HEPES-SDS Running Buffer?
Odpovědět: When using 10x Tris-HEPES-SDS Running Buffer, it’s important to wear appropriate personal protective equipment, such as gloves and goggles, as SDS can be irritating to the skin and eyes. Additionally, ensure to work in a well-ventilated area and dispose of any waste in accordance with local regulations. These precautions help maintain safety and uphold lab standards during protein analysis. -
otázka:
Where can I buy 10x Tris-HEPES-SDS Running Buffer (500 ML) in Czech Republic?
Odpovědět: You can purchase 10x Tris-HEPES-SDS Running Buffer (500 ML) from Ubuy in Czech Republic. Ubuy offers a wide range of laboratory supplies and ensures a seamless shopping experience for researchers and scientists looking for quality products. Their platform allows you to easily find and order this buffer online, ensuring you have the essential reagents for your experiments.
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CZK 1550
Objednejte nyní a položka bude u vás okolo Sobota, červenec 11
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Služby a výhody
- Designed specifically for SDS-PAGE gel electrophoresis.
- Compatible with Thermo Scientific Precise and Pierce Precast Gels.
- Contains essential components: Tris 0.1 M, HEPES 0.1M, SDS 3mM.
- Buffer maintained at optimal pH 8 for performance.
- Easy to store at room temperature or 4°C.
- 500 ML size offers ample supply for multiple experiments.